Saturday, 15 July 2017

Groundwater pumping drying up Great Plains streams, driving fish extinctions


Source: Colorado State University
Summary:
Groundwater pumping from the the High Plains Aquifer has led to long segments of rivers drying up and the collapse of large-stream fishes.

FULL STORY

               The Arikaree River in 2000 in early summer, when water is near its maximum extent.
Credit: Kurt Fausch
                 Farmers in the Great Plains of Nebraska, Colorado, Kansas and the panhandle of Texas produce about one-sixth of the world's grain, and water for these crops comes from the High Plains Aquifer often known as the Ogallala Aquifer the single greatest source of groundwater in North America. A team of researchers, including Colorado State University Professor Kurt Fausch and Jeff Falke, a CSU alumnus and an assistant professor at the University of Alaska Fairbanks, have discovered that more than half a century of groundwater pumping from the aquifer has led to long segments of rivers drying up and the collapse of large-stream fishes.
               If pumping practices are not modified, scientists warn that these habitats will continue to shrink, and the fish populations along with them.
The research team combined modeling from the past and future to assess changes in Great Plains streams and their fish populations associated with groundwater pumping from the High Plains Aquifer. The findings have implications for watersheds around the world, because irrigation accounts for 90 percent of human water use globally, and local and regional aquifers are drying up.
                                                                                                                                                                             A 'train wreck'
               Fausch said the study results are sobering. Based on earlier observations and modeling by Falke and a team of graduate students and faculty at CSU, the Arikaree River in eastern Colorado, which is fed by the aquifer and used to flow about 70 miles, will dry up to about one-half mile by 2045.
"You have this train wreck where we're drying up streams to feed a growing human population of more than 7 billion people," Fausch said.
Fausch described the situation as a "wicked problem," one with no good solution. "More water is pumped out every year than trickles back down into the aquifer from rain and snow," he said. "We are basically drying out the Great Plains."
                                                                                                                                                                   Pumping has dried up streams, small rivers
               Since the 1950s, pumping has extracted nearly as much water as what exists in Lake Erie about 100 trillion gallons and almost none of it trickles back into the aquifer.
"This pumping has dried up long segments of many streams and small rivers in the region," Fausch said. From 1950 to 2010, a total of 350 miles of stream dried up in the large area the team studied in eastern Colorado, southwestern Nebraska and northwestern Kansas. "Our models project that another 180 miles of stream will dry up by 2060," Fausch said.
              The loss of fish in the area is also a concern. "What we're losing are the fishes that require habitat found only in the rivers and large streams of the region, and replacing them with those that can survive in the small streams that are left," Fausch said. "We are losing whole populations of species from rivers in that region because there's no habitat for them."
                As an example, seven of the 16 native fish species that were once found in the Arikaree River have disappeared since the first surveys were done in the 1940s. These fish include small minnows, suckers and catfish, species that the CSU scientist said are not among those that are currently federally endangered or threatened, so there's little regulatory authority to preserve the habitats.
"We're losing fish that people really don't know about," said Fausch. "They are cool and very beautiful, but not charismatic."
                                                                                                                                                                               Losing a river means losing more than fishes
              Effects from the groundwater pumping will extend beyond the fishes and streams, too. Farmers in that area hope to conserve enough water so that future generations can continue to work on the land. And the everyday places that benefit from water could also disappear.
"If they lose the river, they'll not only lose fishes, but they'll also lose water for their cattle, and cottonwoods that provide shade," Fausch explained. "They also lose the grass that grows in the riparian zone, which is critical forage for cattle in summer. Some of that's your livelihood, but it's also the place you go for picnics, and to hunt deer and turkeys. If you lose the river, you lose a major feature of what that landscape is."
               Fausch said that there are some signs of progress, despite the grim findings. Local officials have put meters on wells to ensure that farmers pump only the amount of water allowed under their permits. And farmers are always experimenting with new technology that will allow them to optimize the amount of water they use to achieve the highest crop yields, since it takes electricity to pump the water from deep underground and this is an important cost to them. This doesn't mean that the groundwater levels that feed streams are not declining, but instead are declining at a slower rate than in the past, he said.
                                                                                                                                                               Growing dryland crops an option
               One additional option, though it might be a hard sell, is for farmers to grow dryland crops, meaning that they rely only on rainfall each year, instead of pumping water. The problem is the crop yields then vary widely from year to year, depending on the rain.
"Every farmer understands that eventually they will no longer be able to afford to pump as much water," said Fausch. "Farmers are amazing economists. New options such as economical drip irrigation are being discussed, and farmers will likely switch to these options when they become available."
Fausch, who has studied rivers throughout his entire career, grows wistful when talking about the research. "When we lose these rivers, we will lose them for our lifetime, our children's lifetime, and our grandchildren's lifetime," he said.
Even if all pumping were stopped tomorrow, the aquifer would refill very slowly, over the next 100 years or more, said Fausch. As the groundwater table rose, rivers would start to flow again.

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Squirrels have long memory for problem solving


Source: University of Exeter
Summary:
Squirrels can remember problem-solving techniques for long periods and can apply them to new situations, researchers have discovered.

FULL STORY

                                                         Grey squirrel (stock image).
Credit: © fotogenix / Fotolia
                 Squirrels can remember problem-solving techniques for long periods and can apply them to new situations, researchers have discovered.
University of Exeter scientists found grey squirrels quickly remembered how to solve a problem they had not seen for almost two years.
The squirrels also quickly worked out how to use those skills in a redesigned version of the test.
                  "This might be why grey squirrels can survive very well in towns and cities," said Dr Pizza Ka Yee Chow, of Exeter's Centre for Research in Animal Behaviour.
"For example, they're very good at getting food from bird feeders.
"People may try different types of bird feeders to keep the squirrels away, but this research shows grey squirrels can not only remember tricks for getting food but can apply those skills in new situations."
In the study, five squirrels were given a task identical to one they had tried 22 months earlier, in which they had to press levers to get hazelnuts.
                    In that first experience, the squirrels improved with practice -- taking an average of eight seconds on their first attempt and just two seconds by the final time they tried it.
Trying again for the first time in 22 months, they took an average of just three seconds to get a hazelnut.
Grey squirrels are known to have good long-term memory -- they are "scatter-hoarders," collecting and hiding thousands of nuts every autumn.
"Previous research at Exeter has shown that their memory for the locations of hidden nuts is excellent," said co-author Professor Stephen Lea, of the University of Exeter.
But the new research demonstrates a "very different form of memory," said co-author Dr Théo Robert, also of the University of Exeter.
                  "This is not just remembering where things have been left, it shows they can recall techniques which they have not used for a long time," he said.
"It's also different from what we see in the wild because they're remembering things for longer than the few months of memory needed to find hidden food."
                  When presented with a version of the task that looked different but required the same technique to get hazelnuts, the squirrels showed a "neophobic" (fear of news things) response -- hesitating for more than 20 seconds on average before starting the task.
But once they started it took them just two seconds on average to get a hazelnut, showing that they were able to recall and apply the technique they learned in the previous form of the challenge.
The paper, published in the journal Animal Cognition, is entitled: "How to stay perfect: The role of memory and behavioural traits in an experienced problem and a similar problem."

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Wednesday, 12 July 2017

Chronic liver inflammation linked to Western diet


Food, antibiotics, and gender are just some of the factors that can throw off the balance between the gut and liver, according to a new report in The American Journal of Pathology

Source:
    Elsevier
Summary:
    A new study reports that mice fed a Western diet, which is high in fat and sugar, resulted in hepatic inflammation, especially in males. Moreover, liver inflammation was most pronounced in Western diet-fed male mice that also lacked farnesoid x receptor (FXR), a bile acid receptor.
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FULL STORY
(A) Histology of control diet (CD) and Western diet (WD)-fed wild-type (WT) and FXR knockout (KO) mice of both genders. (B) Histology of antibiotic-treated male mice. Abx is a cocktail of antibiotics that provides broad-spectrum coverage. Vancomycin (Vcm) and polymyxin B (PolyB) cover Gram-positive and negative bacteria, respectively. (C) Relative abundance of cecal microbiota of CD and WD-fed WT as well as FXR KO mice of both genders. (D) Relative abundance of cecal microbiota of antibiotic-treated CD- and WD-fed FXR KO male mice. (Mean values are shown)
Credit: The American Journal of Pathology

A new study in The American Journal of Pathology reports that mice fed a Western diet, which is high in fat and sugar, resulted in hepatic inflammation, especially in males. Moreover, liver inflammation was most pronounced in Western diet-fed male mice that also lacked farnesoid x receptor (FXR), a bile acid receptor.

The study is important because it links diet to changes in the gut microbiota as well as bile acid profile, opening the possibility that probiotics and bile acid receptor agonists may be useful for the prevention and treatment of hepatic inflammation and progression into advanced liver diseases such as cancer.

"We know the transition from steatosis, or fatty liver, to steatohepatitis (inflammation in the fatty liver) plays a crucial role in liver injury and carcinogenesis. Because the liver receives 70% of its blood supply from the intestine, it is important to understand how the gut contributes to liver disease development," explained lead investigator Yu-Jui Yvonne Wan, PhD, Professor and Vice Chair of the Department of Pathology and Laboratory Medicine at UC Davis Health. "Our data show that diet, gender, and different antibiotic treatments alter the gut microbiota as well as bile acid profile and have different effects on liver inflammation."

Dr. Wan and her team used an FXR-deficient mouse model (FXR KO), which has become an important tool to better understand the role of diet and inflammation in the development of liver diseases including cancer because patients with cirrhosis or liver cancer also have low FXR levels.

Other published data have already shown that FXR-deficient mice spontaneously develop steatohepatitis and liver tumors even when they are fed a normal rodent diet. In this study, wild-type and FXR-deficient mice were fed either a Western diet or a matching control diet for 10 months. They found similarities between Western diet intake and FXR deficiency. For instance, both Western diet-fed wild-type mice and control diet-fed FXR KO mice developed steatosis, which also was more severe in males than females. Interestingly, however, only the FXR-deficient male mice had massive lymphocyte and neutrophil infiltration in the liver, and only Western diet-fed male FXR KO mice had fatty adenomas.

"These studies show that a Western diet intake and FXR inactivation also increased hepatic inflammatory signaling, with a combined enhanced effect," Dr. Wan said. "Introducing antibiotics to reduce inflammation also had different effects based on the diets the mice received."

Depending on what type of diet was provided, broad-spectrum antibiotics, which eliminated most gut bacteria, affected hepatic inflammation differently in FXR-deficient mice. In control diet-fed mice, a cocktail of ampicillin, neomycin, metronidazole, and vancomycin completely blocked hepatic neutrophil and lymphocyte infiltration. However, this cocktail of antibiotics (Abx) was not able to eliminate hepatic inflammation in Western diet-fed FXR KO mice. Additional analysis showed that many inflammatory genes had higher expression levels in Western diet than control diet-fed FXR KO mice after Abx treatment.

Analyzing the composition of the gut microbiota, investigators found that Proteobacteria and Bacteroidetes persisted after the broad-spectrum antibiotic treatment in the Western diet-fed FXR KO mice. In contrast, Gram-negative coverage antibiotic, i.e., polymyxin B, increased Firmicutes and decreased Proteobacteria as well as hepatic inflammation in Western diet-fed FXR KO male mice. They suggest that the adverse effects of Western diet on the liver may be explained in part by the persistence of pro-inflammatory Proteobacteria as well as the reduction of anti-inflammatory Firmicutes in the gut.

Primary and secondary bile acids are synthesized by liver cells and gut bacteria, respectively. Bile acids are signaling molecules for lipid and sugar homeostasis as well as inflammatory response. The data generated from this group revealed that the reduced hepatic inflammation by antibiotics was accompanied by decreased free and conjugated secondary bile acids in a gender-dependent manner.

"Gut and liver health are linked. It is clear that microbial imbalance and dysregulated bile acid synthesis are inseparable, and they jointly contribute to hepatic inflammation via the gut-liver axis. In addition, gut microbiota and bile acid profiles may explain gender difference in liver disease as liver cancer incidence is much higher in men than women. Moreover, in antibiotic-treated mice, the change in the profile of bile acids can also be primary as well as secondary to the alterations in gut microbiota because antibiotics can directly eliminate bile acid-generating bacteria, which in turn causes additional changes in the bile acid composition," noted Dr. Wan. "Our results suggest that probiotics and FXR agonists hold promise for the prevention and treatment of hepatic inflammation and progression into advanced liver diseases such as cancer."

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Study sheds light on regulation of hair growth across the entire body


Findings point to new ways of addressing human baldness, unwanted hair

Source:
University of California - Irvine
Summary:
To paraphrase the classic poem, no hair is an island entire of itself. Instead, scientists have discovered that all hairs can communicate with each other and grow in coordination across the entire body. This is regulated by a single molecular mechanism that adjusts by skin region to ensure efficient hair growth - so no bald patches form - and enable distinct hair densities in different body areas.

FULL STORY

Study findings may lead to new ways of addressing both balding and unwanted hair growth.
Credit: © smolaw11 / Fotolia
          To paraphrase the classic poem, no hair is an island entire of itself.
Instead, University of California, Irvine scientists have discovered that all hairs can communicate with each other and grow in coordination across the entire body. This is regulated by a single molecular mechanism that adjusts by skin region to ensure efficient hair growth -- so no bald patches form -- and enable distinct hair densities in different body areas.
In animals, this regulatory process is important for survival in the wild. In humans, these findings could lead to new ways of addressing both balding and unwanted hair growth -- and further understanding of how regions of faster and slower regeneration work in coordination in other fast-renewing tissues, such as the intestines and bone marrow.
           For the study, the researchers used the first mouse model of poor hair growth to analyze human-like hair behavior that leads to baldness. Their results appear in eLife, an open-access journal focusing on the life and biomedical sciences. UCI's Maksim Plikus, assistant professor of developmental & cell biology, and Qing Nie, professor of mathematics, led the effort. Ji Won Oh from Plikus' lab and Qixuan Wang from Nie's lab contributed equally to this work.
                                                                                                                                                                       How skin regions communicate
          The researchers focused on the interaction of the Wnt signaling pathway, which is important in embryonic development and regeneration, and bone morphogenetic proteins, which are hair growth inhibitory factors.
While previous studies have shown that Wnt-BMP signals regulate hair growth in certain body areas, it was not known how different skin regions communicate with one another to coordinate hairs across their borders. By combining expertise in mathematical modeling from Nie's lab and expertise in skin studies from Plikus' lab, Wnt-BMP regulation was found to be ubiquitous across all skin.
"In analogy with languages spoken in two neighboring countries, it was unclear how the back skin 'talks' with the belly skin to coordinate the tasks of growing hairs," Plikus said. "We showed that although different signaling 'dialects' may exist between belly and back skin, for instance, all hairs can understand one another through the use of similar 'words' and 'sentences.'"
                                                                                                                                                                          The roots of hair growth problems
                A breakdown of this complex signaling could uncover the roots of human hair growth irregularities and point to solutions.
For example, common male pattern baldness affects frontal and crown regions but not the back of the head. In adult humans, messaging among scalp hairs appears to stop, and every hair follicle is thought to grow independently.
"If communication between nonbalding and balding regions can be reactivated, hair growth signals can then start spreading across the entire head skin, preventing regional baldness," Plikus said.
"Just like scalp skin can show hair growth deficiency, skin in other body sites -- such as the face, arms and legs -- can often show excessive hair growth that can be cosmetically undesirable," he added. "Our findings suggest that increased signaling crosstalk among hair follicles could be one major reason for this."
                                                                                                                                                                   What's next?
             Plikus said that Wnt and BMP signaling activities can be regulated pharmacologically. "Our study identified the types of Wnt-BMP signaling levels that are very favorable for hair growth and the types that prevent it," he said. "It provides the road map for optimizing Wnt-BMP levels to achieve enhanced hair growth."
He added that the findings point toward additional signaling factors besides Wnt and BMP positively correlated with robust hair growth. Studying these will be the researchers' next step.
Nie noted that laboratory experiments can be insufficient to study complex biological functions, such as hair growth across the entire skin. "In such cases, mathematical modeling can greatly assist in the discovery process," he said. "Our new mathematical model predicted details of signaling communications between hairs, otherwise difficult to reveal with standard biological experiments alone."
 
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New Hampshire growing season for strawberries extended


Source: University of New Hampshire
Summary:
The length of the Granite State's strawberry growing season has been quadrupled as part of a multi-year research project that aims to benefit both growers and consumers.

FULL STORY

          Last year, experiment station researchers harvested strawberries grown in low tunnels for 19 consecutive weeks from mid-July through the week of Thanksgiving.
Credit: Kaitlyn Orde/UNH
           Researchers with the New Hampshire Agricultural Experiment Station at the University of New Hampshire have succeeded in quadrupling the length of the Granite State's strawberry growing season as part of a multi-year research project that aims to benefit both growers and consumers.
New Hampshire's strawberry season traditionally lasts only four to six weeks. However, researchers working on the multi-state TunnelBerries project were picking day-neutral strawberries in Durham last November. Last year, researchers harvested strawberries grown in low tunnels for 19 consecutive weeks from mid-July through the week of Thanksgiving. They also found that the low tunnels significantly increased the percentage of marketable fruit, from an average of about 70 percent to 83 percent. Now in its second year, the TunnelBerries research project is being conducted at the UNH Woodman Horticultural Research Farm. It is part of a larger, multi-state USDA-funded initiative to optimize protected growing environments for berry crops in the upper Midwest and northeastern United States. UNH's component is focused on improving berry quality and the role day-neutral varieties may play in extending the length of strawberry season in the Northeast.
             "Most New Englanders look forward to strawberry season because regionally produced strawberries are delicious," said graduate student Kaitlyn Orde, who is working with experiment station researcher Becky Sideman on the project. "They also are a very valuable early season crop for farmers throughout the region. Unfortunately, though, this season is very brief, limiting the period in which our regional producers are able to meet consumer demand for the fresh fruit. A longer strawberry season is good for both grower and consumer."
Sideman, an associate professor of plant biology and Cooperative Extension professor and specialist in sustainable horticulture production, estimates the retail value of New Hampshire's strawberry crops at about $1.85 million, which she says is a conservative estimate.
The UNH project consists of two parts. Researchers want to determine the yield and fruiting duration of day-neutral strawberry varieties. Day-neutrals are a different plant-type than the traditional June-bearers most common in New Hampshire; day-neutrals (or ever-bearing) have been shown to fruit continuously for four to six months in the region. In addition, day-neutrals fruit the same year they are planted, which is not the case with June-bearers.
            "We are growing one day-neutral variety on three different mulches to determine if there are any differences in total production, production patterns, runner production, and fruit characteristics among the mulches," Orde said. "We also are investigating the role plastic covered low-tunnels play in improving berry quality, and what the microenvironment is within low tunnels, especially late season. To do this, we are evaluating five different plastics for the low tunnels."
Researchers in Maryland, Minnesota, North Carolina, and New York have conducted preliminary research on similar systems. There also are limited growers in the Northeast who already cultivate day-neutral varieties, and even fewer who have experimented with low-tunnels in combination with the strawberry crop. However, there are no yield estimates or material recommendations, such as mulch or low tunnel cover, for New Hampshire producers.

Insect 'anti-antiaphrodisiac' tells males when females are ready to mate


Source: eLife
Summary:
A pheromone has been discovered that is released by female insects after mating that tells males exactly when they are ready to mate again.

FULL STORY

            A Lygus male (right) approaches a recently mated female. He antennates her abdomen to assess her reproductive status. The seminal compounds that have antiaphrodisiac properties are not very volatile, so the male must approach closely.
Credit: Brent et al., 2017
           Researchers have identified a pheromone released by female insects after mating that tells males exactly when they are ready to mate again.
The pheromone, discovered in the western tarnished plant bug Lygus hesperus (L. hesperus), reverses the effects of an antiaphrodisiac found in the male's semen, which stops them from harassing females during the egg-laying period after copulation.
           This so-called 'anti-antiaphrodisiac', described in the journal eLife, adds a new level to the complex communication mechanisms already identified in insect reproduction. Fully understanding these mechanisms could help inform novel control strategies for L. hesperus, a major agricultural pest insect known for its destructive feeding habits.
In many animal species, males guard females to prevent rivals from mating so they can be sure to father the offspring. Such mate guarding can be more passive than active, and can persist even when the male is absent. For example, some male insects transfer an antiaphrodisiac along with their sperm, the smell of which repels other males from inseminated females.
             Previous studies have shown that on the first day of mating, L. hesperus females are less likely to be courted by a male than virgin females due to the effect of a seminal antiaphrodisiac. "However, this effect only persists for a few days," says Research Entomologist Colin Brent from the US Department of Agriculture's Agricultural Research Service, who led the current study. "Over time, those females regain their attractiveness so that, by five days after mating, they are as likely to be courted as virgins. But the mechanism by which this is accomplished has not been determined."
             To discover this, Brent and his colleagues John Byers from the Hebrew University of Jerusalem and Anat Levi-Zada from the Agricultural Research Organization, Volcani Center, Israel -- tested odourants emitted from mated L. hesperus females. Using a technique called gas chromatography mass spectrometry, coupled with behavioural tests, they identified three compounds in the male accessory glands and in capsules containing sperm that were taken from females at various stages after mating.
"Our analyses confirmed the presence of a previously identified antiaphrodisiac called myristyl acetate, and revealed two additional compounds that repel other males from recently mated females," Brent explains. "The female converts one of these internally before releasing it as a third compound, which counteracts the antiaphrodisiac effect caused by the myristyl acetate but does not actually increase male attraction to the female."
             Brent adds that although this is the first recorded instance of an anti-antiaphrodisiac, such compounds may not be rare -- they may have simply been overlooked until now.
"While male Lygus bugs have evolved a chemical mate-guarding mechanism, female evolution favoured a counter mechanism, providing males with an accurate indication of when they are ready to mate again following their egg-laying activity," he says.
"Given the advantages of this two-dimensional chemical signalling system, other examples of anti-antiaphrodisiacs are likely be found. These systems could then be manipulated and turned against the pests, causing them to change their behaviours in a way that could help reduce their population."

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Insecticides damage bee socialization and learning skills, study reports


Source: Taylor & Francis
Summary:
The effects of neonicotinoid insecticides on bees has been widely covered in the news recently, with laboratory-based studies suggesting that the chemicals are harmful, and field studies which are much less clear cut. Adding to current knowledge on the topic, new research further explores the effects that these chemicals may have on social behavior and learning in honey bees.

FULL STORY

                                                                             Bees.
Credit: Image courtesy of Taylor & Francis
             The effects of neonicotinoid insecticides on bees has been widely covered in the news recently, with laboratory-based studies suggesting that the chemicals are harmful, and field studies which are much less clear cut. Adding to current knowledge on the topic, new research published in the Journal of Apicultural Research further explores the effects that these chemicals may have on social behavior and learning in honey bees.
            In one paper, researchers Nadège Forfert and Robin Moritz of Martin Luther University, in Halle-Wittenberg, Germany, explore the effect of the neonicotinoid thiacloprid on social interactions among honey bee workers. They measured interactions in experimental groups of workers to assess the effects of thiacloprid on social network structure, and the amount of food exchanged among worker individuals.
They found that bees fed with thiacloprid significantly reduced their social interactions, suggesting that foraging bees that encounter high doses of insecticide in the field may be less likely to recruit others to these nectar sources, but they also exchanged more food to other group members, which resulted in a dilution of the contaminated food. This means, although thiacloprid may act to interfere with social network structure, it could also play a role in the dynamics of disease transmission in the colony if pathogens are transmitted via food exchange.
             In another paper, Anna Papach and colleagues from the University of Poitier, France look at the effect of exposure of honey bee larvae to the neonicotinoid thiamethoxam and the honey bee brood disease American foulbrood on mortality and cognition. They exposed or co-exposed honey bee larvae to American foulbrood and to sub-lethal doses of thiamethoxam. They found no additive effect between the two stressors on larval mortality, but the results do provide the first evidence of impaired learning and memory in adult bees that had been fed thiamethoxam during the larval stage. They found no alterations in learning and memory in bees after infection with American foulbrood at the larval stage.
Science Director at The International Bee Research Association (IBRA) Norman Carreck says, “These new papers are significant because they fill in some gaps in our knowledge of the effects of neonicotinoid insecticides on social behavior and learning in honey bees. As with previous studies, the question remains as to whether bees experience these effects in the field.”

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